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Laser Microdissection & Pressure Catapulting System:
PALM Microbeam

  Laser_Microdissection_System.gif
 

The Complete System:

Laser microdissection with PALM MicroBeam provides an intelligent tool in molecular analysis at DNA, RNA and protein levels, as it highly improves sampling of cell-specific tissue. Single cells can be captured in a completely contamination-free manner, and pure cells can be pooled1. Even if several hundred cells are needed, LMPC the cutting edge of PALM technology is the solution. The modular set-up of the PALM MicroBeam streamlines the sampling process for downstream microarray, genomics and proteomics work. Depending on the researchers’ needs, captured samples can be processed to individual specifications, thus opening a new dimension to the analysis of gene function. LMPC ensures contamination-free sample collection as the only noncontact technology with sample transport against gravity, and thus paves the way for reliable results

System Components:

  • Axio Observer: Inverted research microscope from Carl Zeiss provides the ‎basis for microdissection. Developed to meet the needs of the most demanding applications. Brilliant optical quality with the high transmission ‎quality and high numerical aperture objectives perfectly suitable for laser microdissection.
  • AxioCam: High sensitive Carl Zeiss digital camera, brilliant for every application
  • PALM RoboMover: Different types of collection vessels can be chosen with the help of PALM RoboMover, individual caps or tubes, 8 Capstrips even microtiter plates.
  • PALM Stage: Stage with the fast drive mechanism for rapid scanning and speedy navigation across the substrate.
  • PALM Control unit: PALM ControlUnit runs and controls the movement of PALM RoboStage, energy- and focus adjustment and the laser. The key-switch also operates the mains for the components of your system. If you want to run single components as for example the computer, it is possible to connect these components directly to the mains.
  • The actual status of the ControlUnit can be observed by LEDs on the front panel. PALM ControlUnit is equipped with up to 4 motor cards, according to the number of motors defined in your system configuration.
  • FTSS laser (Frequency tripled solid state laser): wavelength 355 nm, pulse energy 100 μJ, pulse duration 1 nsec, pulse frequency 100 pulses per second beam divergence 3 mrad, frequency 50/60 Hz, voltage 115/230 V AC

The high beam quality theoretically allows nearly diffraction limited beam focusing. The laser is computer controlled and can be operated either automated with PALM RoboSoftware or manually with the foot switch. It is air-cooled, compact, sturdy, reliable, maintenance-free and requires only a common power supply.

Consumables:

  PALM AdhesiveCaps 200 opaque
415101-4400-240
capture dissected samples without Buffer
Packing Unit: 50 pieces.


PALM AdhesiveCaps 200 clear
415101-4400-245
capture dissected samples without Buffer
Packing Unit: 50 pieces


PALM AdhesiveCaps 500 opaque
415101-4400-250
capture dissected samples without Buffer
Packing Unit: 50 pieces

PALM AdhesiveCaps 500 clear
415101-4400-255
capture dissected samples without Buffer
Packing Unit: 50 pieces


AdhesiveStrips 8C clear
415101-4400-260
capture dissected samples without Buffer
Packing Unit: 12 pieces


AdhesiveStrips 8C opaque
415101-4400-262
capture dissected samples without Buffer
Packing Unit: 12 pieces


CapturePlate 96
415101-4400-291
unfilled, Nuclease and necleic acid free. With certification. For CapturePlate Collector D 96,
Packing Unit: 10 pcs, single packed Lumox Dish 50 (40x5) hydrophil
415101-4400-500 hydrophilic, sterile, 50 pieces


PALM DuplexDish 50
415101-4400-550 Double membrane petriperm dish, sterile packed,
Packing Unit: 10 pieces


PALM DuplexDish 35
415101-4400-551
Double membrane petriperm dish d=35 mm, 2 x 6 pcs. sterile packed
Packing Unit: 12 pieces


PALM MembraneRing 50 (D)
415101-4400-580
PALM MembraneRing 35 415101-4400-581 for microdissection and isolation of living cells with subsequent recultivation used together with routine 35 mm Lumox dishes
Packing Unit: 12 pieces


PALM LiquidCoverGlass N
415101-4400-600
PALM MembraneSlides 1mm
415101-4401-000
PEN membrane-covered, ready to use, with white color marking
Packing Unit: 100 pieces


PALM MembraneSlides 1mm PET
415101-4401-050 PET membrane-covered, ready to use, with blue color marking, four sided sticked,
Packing Unit: 100 pieces.


PALM FrameSlides PET
415101-4401-055
PET-membrane-sided, metal frame Recommended for immunohistochemistry which requires microwave pretreatment or cooking procedures.
Packing Unit: 50 pieces


PALM MembraneSlides 0.17mm
415101-4401-200 PEN membrane-covered, ready to use,
Packing Unit 100 pieces


PALM MembraneSlides 0.17mm
415101-4401-300 POL membrane-covered, ready to use,
Packing Unit: 100 pieces


PALM MembraneSlides 1mm
415101-4401-450 POL membrane-covered, ready to use, with red color marking, four sided sticked,
Packing Unit: 100 pieces


PALM MembraneSlides 0.17mm
415101-4401-500
PEN membrane-covered, ready to use, four sided sticked,
Packaging Unit: 100 pieces


PALM MembraneSlides 0.17mm
415101-4401-550 POL membrane-covered, ready to use, four sided sticked,
Packing Unit 100 pieces


PALM MembraneSlides PEN / NF
415101-4401-600
Nuclease and human Nucleic acid free. With certification.
Single packed 50 pcs.


PALM Demoslides 1mm
415101-4402-010
PEN membrane-covered, ready to use, four sided sticked,
Packing Unit: 5 pieces