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Proteomics Analyzer

Model : 4800 MALDI TOF/TOF Analyzer
Make : Applied Biosystems, USA
Location: Proteomics Lab
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The Complete System:

The 4800 MALDI TOF/TOF Analyzer is the next generation of tandem time-of-flight MS/MS systems, providing the highest level of protein coverage, throughput, and confidence in quantitative proteomic analysis. The 4800 MALDI TOF/TOF is a comprehensive system for proteomics and protein biomarker research.

Overview:

The 4800 MALDI TOF/TOF Analyzer is a floor standing Mass Spectrometer that delivers high resolution and accurate mass determination for the MS analysis of biomolecules including protein digests, intact proteins, lipids, and carbohydrates. The system also enables the precise selection of individual precursor masses for further fragmentation enabling definitive identification and characterization for these biological molecules by MS/MS. Featuring breakthrough optics and powerful, easy-to use software, the versatile 4800 ‎system offers confident, high productivity protein identification and Quantitation for all your discovery applications enabling routine use by expert and non-expert operators.

Applications:

  • Biomarker Discovery
    Protein ID and Quantitation
  • Protein ID
    Includes both MS and MS/MS techniques.
    ID of Protein in gel slices (routine ID from 1D, 2D gels).
    MDLC Fractions and complex samples with LC-MALDI.
  • Protein Expression Analysis
    ID and Quantification of Differen tially expressed proteins (iTRAQTM and ICAT® Reagents)
  • Protein Characterization
    Characterization of phosphorylation and glycosylation peptides by MS & MS/MS techniques. Intact Protein Analysis
  • Carbohydrates, lipids oligosaccharides, etc.

4800 MALDI TOF/TOF Software’s :

4000 Series Explorer Software v3.5: Instrument Control Software, including data acquisition, exclusive tools for LC MALDI analysis using Peak ExplorerTM Software, Data Explorer and Denovo Explorer Software. This software provides access to data, data processing and analysis, method creation, and the ability to submit requests for further analysis remotely to the 4800 MALDI TOF/TOF Analyzer.

GPS Explorer Software v3.5: Innovative software supports many biological workflows such as traditional in-gel digestion, MDLC/MS/MS (LC MALDI), Bio marker Discovery with iTRAQTM Reagents and PTM discovery all with intelligent results dépendant analysis.

A comprehensive set of protein analysis tools including results dependent analysis (RDA) ‎software to remotely submit MS/MS acquisitions based on protein identifications and differential expression results using ICAT Reagents; ID and quantitation in a single multiplexed experiment using iTRAQ Reagents; Support of in-gel digestion and LC MALDI proteomics workflows; Can be used with CDS FASTA files for visualization of protein molecular functions and biological processes directly in ID results with links to the ‎PANTHER (Protein ANalysis THrough Evolutionary Relationships) Classification System.

Pro BLAST Search Engine v3.5: Pro BLAST software enables the performance of BLAST searches on mass spectral-derived peptide data to identify proteins already present in a database, highly modified proteins, or proteins from unsequenced genomes. Can be used with CDS FASTA files for visualization of protein molecular functions and biological processes directly in ID results with links to PANTHER (Protein ANalysis THrough Evolutionary Relationships) Classification System.

Reagents for 4800 MALDI TOF/TOF Analyzer:

iTRAQTM Reagents:
Part No. 4352160 - iTRAQTM reagents Method Development Kit
Contains iTRAQ™ reagents 114, 117, test sample, buffers and reagents to develop a robust enzymatic digestion and peptide labeling protocol for up to three separate analyses.

Part No: 4352135 - iTRAQ™ Reagents Multiplex Kit
Contains iTRAQ™ reagents 114, 115, 116, 117, the appropriate buffers and reagents for 10 two-plex or 6 three-plex or 5 four-plex assays.

Part No: 4352157 – Trypsin 10 pack
Contains sufficient enzyme for 10 duplex, 6 three-plex or 5 four-plex experiments.

ICAT® Reagents:
Part No.4339035 – Cleavable ICAT Method Development Kit – 2 Assays
Cleavable ICAT® reagents Methods Development Kit contains all of the necessary reagents, buffers, cartridges and instructions to start protein expression analysis studies using the new Cleavable ICAT™ reagent technology. The Methods Development kit contains 3 units of heavy reagent and 6 units of light reagent. Each unit of reagent will label approximately 100ug of protein.

Part No.4339036 – Cleavable ICAT Reagent 10 Assay Kit
Part No.4339038 – Cleavable ICAT Reagent Bulk Kit (10 units)
Contains 10 units of both Heavy and light reagent along with Cleaving reagent A and ‎operating instructions. Each unit of reagent will label approximately 100ugs of protein.

Cleaving Reagents:
Part No.4338543 – Cleaving Reagent – A (10 ml for 100x1 unit assay)
Part No.4339052 – Cleaving Reagent – B (0.5ml for 100x 1unit assay))

Cartridges:
Part No.4326694 – Avidin Cartridge 0,2 ml (4,0 mmD x 15 mmL) ( 5 per pack)

Part No.4326695 – Cation Exchange Cartridge 0,2 ml (4,0 mmD x 15 mmL) (5 per pack)

Buffers:
Part No.4326740 – ICAT Avidin Affinity Buffer Pack
Contains individual 100ml bottles of Loading buffer, Wash#1 buffer, Wash #2 buffer, ‎Elution buffer and Storage buffer. The Buffer Pack also includes one 0.2ml Avidin affinity cartridge Part No.4326747 – ICAT Cation Exchange Buffer Pack‎ Contains individual 100ml bottles of Loading buffer, Elution buffer, Cleaning buffer and ‎Storage buffer. The Buffer Pack also includes one 0.2ml Avidin affinity cartridge.

Hardware Kit:
Part No.4326689 – Needle port Adaptor
Part No.4326690 – Outlet Tubing Connector
Part No.4326688 – 0.2ml Cartridge Holder