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TAQMAN Low Density Array Analyzer

Model : ABI 7900HT Low Density Array Analyzer
Make : M/s. Applied Biosystems, USA
Location : Genomics Lab.
TAQMAN Low Density Array Analyzer<

   

TaqMan® Low Density Array. Streamlines reaction setup time, eliminates the need for liquid handling robotics, and provides standardization when screening selected panels of genes.

 

The Applied Biosystems 7900HT Fast Real-Time PCR System is the only real-time quantitative PCR system that combines 384-well plate and Fast real-time PCR capability. With powerful, researcher-friendly software, a 384-well TaqMan® Low Density Array, and Applied Biosystems convenient assay products, it’s easy for labs of all sizes to realize the full potential of this powerful research tool.

Fast, scanning-based detection

The Applied Biosystems 7900HT Fast Real-Time PCR System accommodates higher density plates without compromising speed, resolution, or robust performance. A laser scans and excites the fluorescent dyes in each of the wells; a spectrograph and charge-coupled device (CCD) camera spectrally resolves and collects the fluorescence emission from each sample.

High performance extends to data analysis

The 7900HT system extends high-throughput performance beyond sample analysis by streamlining operation and making results easy to interpret. The intuitive user interface simplifies setup and allows you to quickly analyze sample data and visualize your results for fast, effective decision-making.

Fully automated Enterprise Option

The Enterprise Edition Software included with the 7900HT system, further facilitates high-throughput automated workflows, enables robust, scalable, and secure data management via the included Oracle® database.

384-well TaqMan® Low Density Array saves time and reduces labor-intensive setup.

The TaqMan® Low Density Array is fast and easy to use. It arrives with TaqMan® Gene Expression Assays pre-loaded and lyophilized in the wells. Simply combine your sample with TaqMan® Universal PCR Master Mix and load into the eight card ports. A short centrifugation step transfers the mixes into the individual wells of the card, which is then sealed, and loaded onto the 7900HT system for real-time analysis.

Applied Biosystems microfluidic card technology works together with TaqMan® Gene Expression Assay products help you simply and efficiently analyze tens to hundreds of targets. The convenient, easy-to-use system streamlines the reaction setup process and requires only 24 pipetting steps to fill a 384-well card. No liquid handling robot necessary, and no worries about accuracy of small-volume deliveries. The low-volume design minimizes sample and reagent consumption, delivering more information per sample than the traditional 96-well or 384-well formats.

Applications

Your choice of quantitation methods

The 7900HT Fast Real-Time PCR System gives you a choice of quantitation methods for gene expression. The absolute quantitation method determines target quantity directly from a standard curve. Relative quantitation eliminates the need for a standard curve by calculating quantitation values of an unknown sample relative to a calibration sample. Either way, the software completely automates data analysis, and can automatically set the optimal baseline for each well, as well as the optimal threshold for each primer and probe set (TaqMan® assays) or assay-specific primer set (SYBR® Green Dye).

Absolute quantitation

To quantitate the amount of target in unknown samples, the 7900HT system measures the sample’s CT* and uses a standard curve to determine starting copy number. To prepare the standard curve, the 7900HT system software first calculates CT values from dilutions of samples with known starting copy number. The software then plots the log of the starting copy number against the measured CT value. The relationship between CT and starting copy number is linear over at least five orders of magnitude.

Relative Quantitation

Relative quantitation is ideal for gene expression studies. Instead of using a standard curve, expression levels are calculated relative to a calibration sample—saving you time and money. An endogenous control is used to normalize the sample amount. This method, coupled with the ability of the 7900HT system to quantitate both the target and endogenous control in a multiplex reaction, enables highly cost-effective, high-throughput gene expression studies. The relative quantitation (RQ) study, which comes standard with the 7900HT system, allows simultaneous analysis of up to ten plates of data.

A complete system for SNP genotyping

In addition to real-time quantitation, the 7900HT system includes software for large-scale screening of known SNPs. In a two-allele system, TaqMan® probes for each allele are multiplexed in a single tube. End-point fluorescence is measured by the 7900HT system, and experimental results are rapidly generated by the integrated data analysis software.

Simplify your workflow, increase your confidence

Alternate analysis techniques require numerous steps and manual intervention. By contrast, the 5> nuclease assay chemistry calls for already-familiar tools and requires no complicated sample preparation. Simply pipette genomic DNA or cDNA, TaqMan® Universal PCR Master Mix or TaqMan® Fast Universal PCR Master Mix for fast cycling, and your assay probe and primer mix into microplate wells for real-time cycling or end-point analysis on the 7900HT system

Reagent Ordering Information

Absolute and Relative Quantitation

Item Description Part Number No. of Reactions
TaqMan® Fast Universal PCR Master Mix (2?), No AmpErase® UNG 4352042 250
TaqMan® Fast Universal PCR Master Mix, No AmpErase® UNG, 5-Pack 4366073 1250
TaqMan® Gene Expression Master Mix, 1-Pack 4369016 200
TaqMan® Gene Expression Master Mix, 2-Pack 4369514 400
TaqMan® Gene Expression Master Mix, 5-Pack 4369510 1000
TaqMan® Universal PCR Master Mix, No
AmpErase® UNG, 2 x 200 Reactions
4364341 400
Power SYBR ® Green PCR Master Mix, 5 ml (1-Pack) 4367659 200
Power SYBR® Green PCR Master Mix, 2 x 5 ml (2-Pack) 4368706 400
High Capacity cDNA Reverse Transcription Kit with RNAse inhibitor, 4374966 200
High Capacity cDNA Reverse Transcription Kit with RNAse inhibitor, 4374967 1000
TaqMan® Human RNase P Control Reagents Kit 4316844 1000
TaqMan® Human RNase P Detection Reagent Kit 4316831 100
TaqMan® Human GAPDH Control Reagents (JOE TM dye) 402869 100
TaqMan® Exogenous Internal Positive Control Reagents (VIC ® dye) 4308323 200
MicroAmp ® Optical 384-well reaction plate with Barcode (code 128) 4309849 50 plates
MicroAmp TM Optical Adhesive Cover Starter Kit 4313663 25 films

TaqMan Low Density Array Panels

Item Description Part Number No. of Reactions
TaqMan® Low-Density Array for Gene Expression; Format 12 4342247 1 Array
TaqMan® Low-Density Array for Gene Expression; Format 24 4342249 1 Array

Item Description Part Number No. of
 Reactions
7900HT TaqMan ® Low Density Array for Gene Expression;
Format 32
4346799 1 Array
Taqman ® Low-Density Array for Gene Expression;
Format 48
4342253 1 Array
Taqman ® Low-Density Array, Human Endogenous Control Panel 4367563 2 arrays
Taqman ® Low-Density Array, Human ABC Transporter Panel 4378700 4 / Pack
Taqman ® Low-Density Array, Human Alzheimer’s Panel 4378713 4 / Pack
Taqman ® Low-Density Array, Human Apoptosis Panel 4378701 4 / Pack
Taqman ® Low-Density Array, Human Immune Panel 4370573 4 / Pack
Taqman ® Low-Density Array, Human Inflammation Panel 4378707 4 /Pack
Taqman ® Low-Density Array, Human Stem Cell Pluripotency Panel 4385344 4 / Pack
Taqman ® Low-Density Array, Mouse GPCR Panel 4378703 4 / Pack
Taqman ® Low-Density Array, Rat GPCR Panel 4378709 4 / Pack
Taqman ® Low-Density Array, Rat Inflammation Panel 4378708 4 / Pack